Synchronization of Dictyostelium discoideum adhesion and spreading using electrostatic forces.
Identifieur interne : 001716 ( Main/Exploration ); précédent : 001715; suivant : 001717Synchronization of Dictyostelium discoideum adhesion and spreading using electrostatic forces.
Auteurs : RBID : pubmed:20472511English descriptors
- KwdEn :
- Actins (metabolism), Cell Adhesion (physiology), Cell Movement (physiology), Dictyostelium (cytology), Dictyostelium (physiology), Electrochemistry, Electrophysiological Phenomena, Fluorescence, Green Fluorescent Proteins (analysis), Intracellular Membranes (metabolism), Microscopy, Interference, Static Electricity, Tin Compounds (chemistry).
- MESH :
- chemical , analysis : Green Fluorescent Proteins.
- chemical , chemistry : Tin Compounds.
- chemical , metabolism : Actins.
- cytology : Dictyostelium.
- metabolism : Intracellular Membranes.
- physiology : Cell Adhesion, Cell Movement, Dictyostelium.
- Electrochemistry, Electrophysiological Phenomena, Fluorescence, Microscopy, Interference, Static Electricity.
Abstract
Synchronization of cell spreading is valuable for the study of molecular events involved in the formation of adhesive contacts with the substrate. At a low ionic concentration (0.17 mM) Dictyostelium discoideum cells levitate over negatively charged surfaces due to electrostatic repulsion. First, a two-chamber device, divided by a porous membrane, allows to quickly increase the ionic concentration around the levitating cells. In this way, a good synchronization was obtained, the onsets of cell spreading being separated by less than 5 s. Secondly applying a high potential pulse (2.5 V/Ref, 0.1s) to an Indium Tin Oxide surface attracts the cells toward the surface where they synchronously spread as monitored by LimE(Deltacoil)-GFP. During spreading, actin polymerizes in series of active spots. On average, the first spot appears 8-11s after the electric pulse and the next ones appear regularly, separated by about 10s. Synchronized actin-polymerization activity continues for 40s. Using an electric pulse to control the exact time point at which cells contact the surface has allowed for the first time to quantify the cellular response time for actin polymerization. Electrochemical synchronization is therefore a valuable tool to study intracellular responses to contact.
DOI: 10.1016/j.bioelechem.2010.04.003
PubMed: 20472511
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Synchronization of Dictyostelium discoideum adhesion and spreading using electrostatic forces.</title><author><name sortKey="Socol, Marius" uniqKey="Socol M">Marius Socol</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI), Grenoble Institute of Technology, 1130 Rue de la Piscine, BP 75, 38402 Saint-Martin d'Hères Cedex, France. m_socol2000@yahoo.com</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI), Grenoble Institute of Technology, 1130 Rue de la Piscine, BP 75, 38402 Saint-Martin d'Hères Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Rhône-Alpes</region><settlement type="city">Saint-Martin d'Hères</settlement></placeName></affiliation></author><author><name sortKey="Lefrou, Christine" uniqKey="Lefrou C">Christine Lefrou</name></author><author><name sortKey="Bruckert, Franz" uniqKey="Bruckert F">Franz Bruckert</name></author><author><name sortKey="Delabouglise, Didier" uniqKey="Delabouglise D">Didier Delabouglise</name></author><author><name sortKey="Weidenhaupt, Marianne" uniqKey="Weidenhaupt M">Marianne Weidenhaupt</name></author></titleStmt><publicationStmt><date when="2010">2010</date><idno type="doi">10.1016/j.bioelechem.2010.04.003</idno><idno type="RBID">pubmed:20472511</idno><idno type="pmid">20472511</idno><idno type="wicri:Area/Main/Corpus">001932</idno><idno type="wicri:Area/Main/Curation">001932</idno><idno type="wicri:Area/Main/Exploration">001716</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actins (metabolism)</term><term>Cell Adhesion (physiology)</term><term>Cell Movement (physiology)</term><term>Dictyostelium (cytology)</term><term>Dictyostelium (physiology)</term><term>Electrochemistry</term><term>Electrophysiological Phenomena</term><term>Fluorescence</term><term>Green Fluorescent Proteins (analysis)</term><term>Intracellular Membranes (metabolism)</term><term>Microscopy, Interference</term><term>Static Electricity</term><term>Tin Compounds (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Tin Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Actins</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Dictyostelium</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Intracellular Membranes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Adhesion</term><term>Cell Movement</term><term>Dictyostelium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electrochemistry</term><term>Electrophysiological Phenomena</term><term>Fluorescence</term><term>Microscopy, Interference</term><term>Static Electricity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Synchronization of cell spreading is valuable for the study of molecular events involved in the formation of adhesive contacts with the substrate. At a low ionic concentration (0.17 mM) Dictyostelium discoideum cells levitate over negatively charged surfaces due to electrostatic repulsion. First, a two-chamber device, divided by a porous membrane, allows to quickly increase the ionic concentration around the levitating cells. In this way, a good synchronization was obtained, the onsets of cell spreading being separated by less than 5 s. Secondly applying a high potential pulse (2.5 V/Ref, 0.1s) to an Indium Tin Oxide surface attracts the cells toward the surface where they synchronously spread as monitored by LimE(Deltacoil)-GFP. During spreading, actin polymerizes in series of active spots. On average, the first spot appears 8-11s after the electric pulse and the next ones appear regularly, separated by about 10s. Synchronized actin-polymerization activity continues for 40s. Using an electric pulse to control the exact time point at which cells contact the surface has allowed for the first time to quantify the cellular response time for actin polymerization. Electrochemical synchronization is therefore a valuable tool to study intracellular responses to contact.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">20472511</PMID><DateCreated><Year>2010</Year><Month>08</Month><Day>03</Day></DateCreated><DateCompleted><Year>2010</Year><Month>12</Month><Day>17</Day></DateCompleted><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1878-562X</ISSN><JournalIssue CitedMedium="Internet"><Volume>79</Volume><Issue>2</Issue><PubDate><Year>2010</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Bioelectrochemistry (Amsterdam, Netherlands)</Title><ISOAbbreviation>Bioelectrochemistry</ISOAbbreviation></Journal><ArticleTitle>Synchronization of Dictyostelium discoideum adhesion and spreading using electrostatic forces.</ArticleTitle><Pagination><MedlinePgn>198-210</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.bioelechem.2010.04.003</ELocationID><Abstract><AbstractText>Synchronization of cell spreading is valuable for the study of molecular events involved in the formation of adhesive contacts with the substrate. At a low ionic concentration (0.17 mM) Dictyostelium discoideum cells levitate over negatively charged surfaces due to electrostatic repulsion. First, a two-chamber device, divided by a porous membrane, allows to quickly increase the ionic concentration around the levitating cells. In this way, a good synchronization was obtained, the onsets of cell spreading being separated by less than 5 s. Secondly applying a high potential pulse (2.5 V/Ref, 0.1s) to an Indium Tin Oxide surface attracts the cells toward the surface where they synchronously spread as monitored by LimE(Deltacoil)-GFP. During spreading, actin polymerizes in series of active spots. On average, the first spot appears 8-11s after the electric pulse and the next ones appear regularly, separated by about 10s. Synchronized actin-polymerization activity continues for 40s. Using an electric pulse to control the exact time point at which cells contact the surface has allowed for the first time to quantify the cellular response time for actin polymerization. Electrochemical synchronization is therefore a valuable tool to study intracellular responses to contact.</AbstractText><CopyrightInformation>2010 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Socol</LastName><ForeName>Marius</ForeName><Initials>M</Initials><Affiliation>Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces (LEPMI), Grenoble Institute of Technology, 1130 Rue de la Piscine, BP 75, 38402 Saint-Martin d'Hères Cedex, France. m_socol2000@yahoo.com</Affiliation></Author><Author ValidYN="Y"><LastName>Lefrou</LastName><ForeName>Christine</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Bruckert</LastName><ForeName>Franz</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Delabouglise</LastName><ForeName>Didier</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Weidenhaupt</LastName><ForeName>Marianne</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>04</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Bioelectrochemistry</MedlineTA><NlmUniqueID>100953583</NlmUniqueID><ISSNLinking>1567-5394</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Actins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Tin Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance>Green Fluorescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>71243-84-0</RegistryNumber><NameOfSubstance>indium tin oxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Actins</DescriptorName><QualifierName MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Adhesion</DescriptorName><QualifierName MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Cell Movement</DescriptorName><QualifierName MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Dictyostelium</DescriptorName><QualifierName MajorTopicYN="Y">cytology</QualifierName><QualifierName MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Electrochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Electrophysiological Phenomena</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Intracellular Membranes</DescriptorName><QualifierName MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Microscopy, Interference</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y">Static Electricity</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Tin Compounds</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>9</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2010</Year><Month>4</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>4</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2010</Year><Month>4</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>5</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>5</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>12</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">S1567-5394(10)00070-8</ArticleId><ArticleId IdType="doi">10.1016/j.bioelechem.2010.04.003</ArticleId><ArticleId IdType="pubmed">20472511</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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